“expressed sequence tag”的概念、定义、翻译、参考文献-科学参考

expressed sequence tag

Biology

A short partial sequence, typically 200–400 bp long, of a complementary DNA (cDNA) clone. Because cDNAs are prepared by reverse transcription of messenger RNA molecules, ESTs act as markers for genes that are expressed in particular tissues or organs. EST sequence data are held on databases, and researchers use computer programs to search for sequences that correspond to, say, a partial amino-acid sequence for a protein under investigation. The EST sequence can then be used to construct a PCR primer or DNA probe to locate the respective clone from a DNA library.

单词	expressed sequence tag
释义	expressed sequence tag Biology A short partial sequence, typically 200–400 bp long, of a complementary DNA (cDNA) clone. Because cDNAs are prepared by reverse transcription of messenger RNA molecules, ESTs act as markers for genes that are expressed in particular tissues or organs. EST sequence data are held on databases, and researchers use computer programs to search for sequences that correspond to, say, a partial amino-acid sequence for a protein under investigation. The EST sequence can then be used to construct a PCR primer or DNA probe to locate the respective clone from a DNA library.
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