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单词 variable number tandem repeats
释义
variable number tandem repeats

Biology
  • A family of genetic loci found in eukaryotes consisting of short (15–100 bp) sequences of DNA repeated in tandem arrays; in humans these arrays are typically 1–5 kb long. The alleles for any particular locus all have the same sequence but differ as to how many times the sequence is repeated. VNTR loci contribute to repetitive DNA and have proved valuable in DNA profiling. The VNTR sequences can be released intact from a DNA sample using restriction endonuclease enzymes and identified using gene probes with Southern blotting. Alternatively, the VNTR alleles can be amplified by the polymerase chain reaction, separated by gel electrophoresis, and the resultant patterns compared without the need for special gene probes. Since each VNTR locus typically has many different alleles, the likelihood of two individuals having identical sets of alleles for even a few such loci is very remote. For forensic purposes, VNTRs have now been supplanted by short tandem repeats.


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