A graphical method used to obtain a straight line from experimental data from enzyme kinetics (see Fig. 18). It involves forming a plot of V/S versus V in which S is the substrate concentration at which the velocity v is observed. The gradient of the line is equal to –K_m and the intercept on the y-axis is equal to the maximum velocity V. It is named after Canadian biochemist George Sharp Eadie (1895–1976) and B. H. J Hofstee who developed the plot in 1942 and 1959, respectively. See michaelis–menten kinetics.

Fig. 18