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等位基因特异性核苷酸掺入
释义
等位基因特异性核苷酸掺入
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分子杂交
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allele-specific nucleotide incorporation
等位基因特异性核苷酸掺入
利用引物3′端与单核苷酸多态性(SNP)位点的匹配程度来控制引物的延伸,引物3′端正好位于SNP位点,设计两条引物分别使其3′端与SNP的两种碱基互补,根据延伸反应的“有无”信号来判断SNP类型的方法。又称等位基因特异聚合酶链反应(AS-PCR)。
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