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微球细胞固定法
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微球细胞固定法
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microsphere cell immobilization
微球细胞固定法
通过物理或者化学方法将游离的单个细胞固定在单个固体微球上,在适合的条件下培养一段时间,使微球表面的细胞生长成一个微群落,将微球表面固定的细胞与显色底物或者荧光底物进行反应,用显微镜或其他仪器检测信号变化,或者与数字成像系统结合使用,从而筛选、分离、回收目标突变体的方法。
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