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多肽固相合成
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多肽固相合成
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医学
多肽固相合成
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peptide synthesis in solid phase
多肽固相合成
多肽的化学合成方法,先将要合成的链C端氨基酸的羧基以共价键与不溶性的高分子树脂相连,然后,以此氨基酸作为氨基组分经过脱去氨基保护基,并与过量活化的羧基组分反应延长肽链,重复操作(缩合-洗涤-去保护-中和洗涤-下一轮缩合),达到要合成的肽链长度,最后将肽链从树脂上裂解下来,经过纯化等处理,得到目标多肽。
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