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双分子荧光互补技术
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双分子荧光互补技术
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定量荧光成像
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bimolecular fluorescence complementation; BiFC
双分子荧光互补技术
直观、快速地判断目标蛋白在活细胞中的定位和相互作用的技术。该技术巧妙地将荧光蛋白分子的两个互补片段分别与两个目标蛋白融合表达,如果荧光蛋白的发光活性恢复则表明两目标蛋白发生了相互作用。
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