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双分子荧光互补
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双分子荧光互补
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bimolecular fluorescence complementation,BiFC
双分子荧光互补
将两个蛋白质分别与荧光蛋白的N端片段和C端片段相连表达成两个融合蛋白,利用蛋白质片段互补原理,通过检测荧光强度验证这两个蛋白质相互作用的技术。
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