“Sanger测序”的概念、定义、翻译、参考文献-科学参考

Sanger测序

Encyclopedia

医学Sanger测序释
Sanger sequencingSanger测序
单链脱氧核糖核酸（DNA）模板在DNA聚合酶、引物、四种脱氧核糖核苷酸（dNTP，即dATP、dCTP、dGTP和dTTP）存在的条件下进行新链合成，当双脱氧核糖核苷酸（ddNTP，即ddATP、ddCTP、ddGTP和ddTTP）加入正在延伸的DNA链时，由于ddNTP没有延伸所需要的3'-OH基团，双脱氧核糖核苷酸将阻断DNA链延伸，从而获得长短不一的反应产物，通过电泳分离，从四条泳道上的条带顺序就能读出DNA序列的测序方法。又称末端终止法测序技术。

单词	Sanger测序
释义	Sanger测序 Encyclopedia 医学Sanger测序释 Sanger sequencingSanger测序单链脱氧核糖核酸（DNA）模板在DNA聚合酶、引物、四种脱氧核糖核苷酸（dNTP，即dATP、dCTP、dGTP和dTTP）存在的条件下进行新链合成，当双脱氧核糖核苷酸（ddNTP，即ddATP、ddCTP、ddGTP和ddTTP）加入正在延伸的DNA链时，由于ddNTP没有延伸所需要的3'-OH基团，双脱氧核糖核苷酸将阻断DNA链延伸，从而获得长短不一的反应产物，通过电泳分离，从四条泳道上的条带顺序就能读出DNA序列的测序方法。又称末端终止法测序技术。
随便看	direct economic loss of transportation accident directed directed acyclic graph directed acyclic graph, DAG directed acyclic graphical model directed angle directed arc directed assembly directed beam directed-beam directed broadcast address directed circuit directed closed path directed complete graph directed complete partial order directed complete partial order, DCPO directed convex polyomino directed cycle directed design directed diffusion directed distance directed downward set directed ear directed edge directed-edge sequence