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Sanger测序
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Sanger测序
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Sanger测序
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Sanger sequencing
Sanger测序
单链脱氧核糖核酸(DNA)模板在DNA聚合酶、引物、四种脱氧核糖核苷酸(dNTP,即dATP、dCTP、dGTP和dTTP)存在的条件下进行新链合成,当双脱氧核糖核苷酸(ddNTP,即ddATP、ddCTP、ddGTP和ddTTP)加入正在延伸的DNA链时,由于ddNTP没有延伸所需要的3'-OH基团,双脱氧核糖核苷酸将阻断DNA链延伸,从而获得长短不一的反应产物,通过电泳分离,从四条泳道上的条带顺序就能读出DNA序列的测序方法。又称末端终止法测序技术。
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