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RNA印迹法
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RNA印迹法
Encyclopedia
理学
核酸
释
Northern blotting
RNA印迹法
通过凝胶电泳分离RNA,再将其转移到杂交膜上,并用带有标记的探针进行检测的常用的RNA分子杂交检测方法。可用于检测基因表达和转录本长度,分析可变剪接等。
理学
基因工程
释
Northern blotting
RNA印迹法
在变性条件下利用琼脂糖凝胶将RNA分离并进行尼龙膜或者硝酸纤维素膜转印后,再用放射性标记的探针依据其同源性对目的RNA分子进行杂交检测的方法(见图)。
Biology
Northern blotting
RNA印迹法
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