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megaprimer PCR
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megaprimer PCR
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理学
酶定向进化
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megaprimer PCR
大引物聚合酶链反应法
利用一条含突变的引物和一条普通引物对DNA模板进行扩增得到DNA短片段,并以此短片段为引物,再次进行聚合酶链反应(PCR)扩增得到含点突变的完整基因的两步PCR点突变构建方法。
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