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双探针杂交技术
使用两种不同荧光标记的杂交探针与目的片段结合,在结合过程中,当供体荧光基团与受体荧光基团靠近时,通过荧光共振能量转移使受体荧光被激发,产生的荧光被仪器读取的实时定量聚合酶链反应(PCR)技术。
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