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gene site-directed integration
释义
gene site-directed integration
Encyclopedia
理学
基因定点整合
释
gene site-directed integration
基因定点整合
构建含有同源序列的DNA片段的整合载体,通过各种转化方法,使外源DNA序列与靶序列之间发生同源重组,从而将靶DNA序列定点破坏,通过一系列筛选手段,最终得到定向转化的细胞的过程。又称基因打靶、定向基因转移。
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